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Sequences within the LBD form the binding site for hsp90 that blocks the DBD in the cytosolic, nonliganded GR. The C-terminal AF-2 transactivation domain is highly conserved within the nuclear receptor superfamily 36 and is recognized by various transcriptional coregulators, formerly referred to as coactivators or corepressors, clenbuterol tummy shots. In ERα it constitutes aa 530–553. Although individual domains of steroid/nuclear receptors can be exchanged 70 and function when spliced with nonrelated transcription factors, 71 forming chimeric proteins, experiments on ERα 72 and GR 73 show that these receptors function optimally when intact.
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Activated protein kinases initiate a phosphorylation cascade , in which multiple protein kinases phosphorylate (add a phosphate group to) numerous and various cellular proteins, including other enzymes (Step 6). These hormones must bind to a surface cell-membrane receptor, . The receptor then initiates a cell-signaling pathway within the cell involving G proteins, adenylyl cyclase, the secondary messenger cyclic AMP (cAMP), and protein kinases. In the final step, these protein kinases phosphorylate proteins in the cytoplasm.Clenbuterol tummy shots, prednisone for tendonitis in elbow 
 
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